Part:BBa_K2768006:Design

Pnao

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1170
  Illegal BamHI site found at 1278
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 120
  Illegal NgoMIV site found at 465
  Illegal NgoMIV site found at 1083
  Illegal AgeI site found at 784
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 424
  Illegal BsaI.rc site found at 688
  

Design Notes

We did the codon optimization, referring to E.coli K12

Source

Pnao sequence https://www.ncbi.nlm.nih.gov/protein/WP_044040031.1?report=genpept; Pseudomonas putida S16 (strain: S16) genomic sequence: https://www.ncbi.nlm.nih.gov/nuccore/NC_015733.1?report=genbank